Laboratory equipment bd facsjazz user manual 9 pages. Analysis and sorting of cells with facsaria ii flow cytometer 10. Bd facsaria ii is quite easy to set up but requires a lot of maintenance work. For a full description of the cytometer status report, see the bd facsdiva software reference manual. Further details can be found in the reference manual, provided on the documentation cd, for either the bd facscanto or the bd facscanto ii flow cytometer. It accommodates up to six aircooled lasers and eighteen fluorescent detectors. Bd facscanto ii instructions for use manual pdf download. If you are not familiar with the windows operating system, s ee the documentation provid ed with your computer. The expert staff at nyu langones cytometry and cell sorting laboratory can assist you in using a facsaria iiu sorp cell sorter. Operate the instrument only as directed in the users guide. For detailed information about preparing samples for the bd facs aria ii, see. Analysis and sorting of cells with facsaria ii flow cytometer tiina pessamorikawa revised 20181023 the instruction refers to the operating manual faug, i. Bd facsariaii has fixedalignment cuvette flow cell, which allows achieving superior fluorescence sensitivity. If needed, select a different sort setup from the sort menu.
Verify that the air and fluid lines are disconnected from the ethanol tank y. Lsr ii and lsrfortessa are operated using the same software, facsdiva. Lasers not required for analysis andor sorting need to. Bd facsaria flow cytometer is a class i laser product per 21 cfr 28 mar 2016 check that the sheath tank is full and the waste tank is empty. Whisper aerosol management unit this unit is supplied by the facsaria cytometer manufacturer bd biosciences to remove and filter aerosols from the cytometers interior. Page 1 use this quick reference guide once you are familiar with the procedures in the bd facsaria ii users guide. Page 1 bd facscanto ii instructions for use for in vitro diagnostic use part no.
Improved indicators in the browser to show where sort layouts are being created. Pause counters to load a new tube into the bd facsaria cell sorter. The bd facsaria iii user s guide assumes you have a bd facsdiva software quick reference guide for bd facsaria cell sorters. Bd facsaria fusion manual 227 amici del golden retriever. Comparison of sorting capabilities of beckman coulter moflo. Facsaria sorp standard operation protocol basic operation. Starting up the cytometer follow these steps to start up your bd facsaria cytometer. Bd facsaria i operation manual 1 facsaria i standard operation protocol basic operation 1. A copy is at the end of this manual, for the new user to be certified to enter the core or work independently in the facility. Safety manual and standard operating procedures for the ocs flow cytometry core new york university.
The bd facsaria ii users guide assumes you have a working knowledge of basic microsoft. The instrument is able to perform two and fourway aseptic sorting for a variety of tube sizes as well as sorting into multiwell plates. Bd facsaria iii addendumto the bd facsaria ii users guide. Masks divide cells into 32 equal segments, and choosing different types of available masks determines how drops will be deflected if a sorting conflict occurs bd biosciences facsaria training manual. The university of washingtons surplus property office is pleased to offer this bd facsaria ii cell sorter system. A june 2007 bd biosciences benex limited canada japan bay k 1 ad nippon becton dickinson 2350 qume drive toll free 888 2590187 company, ltd. Log in to the computer facsuser, password bdis and open bd facsdiva software. Moflo xdp instructions for use, fullerton, california.
For cell analysis, we can use minimum amount of cells for antibody staining and still get good reading. A bd facsaria iii cytometer report includes the following additional information. Bd facsaria ii system makes cell sorting even easier and more accessible. Facsaria ii mirror filter fluorochrome examples detector 488 690lp 69540 percpcy55 a 200mw 505 lp 52550 fitc,gfp, af488, cfse, pkh67 b 48810 ssc c 405 570lp 57525 bv570, qdot 585 a 100mw 505lp 52550 bv500, amcyan, cfp, pacific orange b. Cytometry tube load errors on facsaria iiu sorp and facsaria iisorp.
Aug 25, 2017 this video describes the process of manual and auto compensation in bd facsdiva on the lsr ii. Decontamination of the bd facsaria ii system using the prepare for aseptic sort procedure. This capability makes the bd facsaria platform the best possible choice and a. Cytometry tube load errors on facsaria iiu sorp and facsaria iisorp cris bare flowmail at thu jul 11. Bd facsaria or bd facsaria ii system up to the capabilities of the bd facsaria iii. The bd facsaria is new generation benchtop highspeed flow cytometry cell sorter. Bd facscanto ii measuring instruments user manual manual pdf. Please, provide a brief description of your project and its aim. Addition of experiment and specimen information to sort reports. Sufficient time 30 minutes need to be given to allow the lasers to warm up completely. Cytometry tube load errors on facsaria iiu sorp and. Page 1 use this quick reference guide once you are familiar with the procedures in the bd facsaria ii user s guide.
Bd facsaria user guide bd facsaria ii has three lasers. The new bd facsaria ii cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. For applicatio nspecific instructions, refer to the individual. Facsaria ii the bd facsaria is a color 15parameter custommade highspeed digital benchtop cell sorter equipped with four adjustable excitation lasers 100 mw blue, 50 mw 561nm green, 100 mw red and 100 mw violet. Bd facscanto ii measuring instruments user manual manual. A mask is a region of the stream which is monitored for the presence of cells of interest defined by bd biosciences facsaria training manual. Bd facsaria users guide national center for xray tomography. The expert staff at nyu langones cytometry and cell sorting laboratory provides researchers with training on and access to a sp6800 spectral analyzer. Patented bd facs accudrop technology assists the user to see the best drop delay value which is invisible to the naked eye. Aria is the one of the best cell sorter flowcytometer. Facsaria ii users guide and page number for additional information. Starting up the cytometer follow these steps to start up your bd facsaria ii cytometer. Each user is responsible for backing up hisher data files. This facsaria ii was manufactured in march of 2008 and has three 3 lasers.
Bd facsaria ii users guide the bd facsaria ii cytometer uses lowpowered, aircooled and solidstate lasers that do not have special power or cooling requirements. Our facsaria ii is a 5laser cell sorter that can measure at the same time up to 18 fluorescence parameters and 2 light scatter parameters. Laboratory equipment bd facsort user manual 14 pages. Although this manual has been prepared with every precaution to ensure accuracy, bd biosciences assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information. The bd facsaria ii system was set up for a sort using either a 70.
Cytometry tube load errors on facsaria iiu sorp and facsaria iisorp next message. Equipment documentation applied precision deltavision core deltavision core specs olympus ix71 microscope stand users manual photometrics coolsnap hq2 weatherstation environmental chamber manual bd facsaria ii flow cytometer bd facsaria ii website facsaria ii tech specs bdcytopeia influx flow cytometer influx user guide isb influx quickstart operation isb influx operator manual. For more details, refer to chapter 4 in the users guide. Bd facsaria ii users guide indiana university bloomington.
Bd facsariaii delivers highspeed multicolor sorting with digital acquisition rates of up to 20,000 eventssecond. Designed with input from customers around the world, the new bd facsaria ii improves ease of use, flexibility, and aseptic capability to make it simply the best choice for consistent results in sorting across a broad range of applications. Keep this safety information available for future reference and for new users. The procedures outlined in this sop are a supplement to general laboratory safety procedures outlined in the uw biosafety manual. References bd facsaria ii users guide, part number 643245, rev a, 2007 for research use only. The bd facsaria cell sorter sets a new standard for highperformance flow cytometry. The facsaria ii is under an annual service contract and receives two quality control service calls per year. For detailed information on software features, see the bd facsdiva. Compensation of a 7 color panel on the bd lsr ii youtube. In addition to being placed in a hood, this instrument has an aerosol containment system. The following figure shows the steps for daily workflow using the bd facsaria user s guide assumes you have a working knowledge of basic.
The facsaria iiiu, or aria as we more commonly call it, is a fourlaser cell sorter. Human regulatory tcell isolation and measurement of function. Laser safety lasers or laser systems emit intense, coherent electromagnetic radiation that. Bd facsaria ii quick reference manual pdf download. Its primary function is to examine complex populations of cells and yield pure populations of cells. Lasers not required for analysis andor sorting need to be switched off. The aria has four lasers, 405, 488, 561 and 633 nm, and can detect light in sixteen photomultiplier tubes.
Lsr ii has been recently upgraded with a violet laser with 6 detectors and an additional detector off the red laser for alexa fluor 700. Check that the sheath tank is full and the waste tank is empty. Users guide the bd facsaria users guide assumes you have a working knowledge of basic. This guide contains instructions for using bd facsdiva software version 6. Built on fixedalignment technology as in the firstgeneration bd facsaria system, the flow cell of the bd facsaria ii is in true fixed alignment with the laser, to reduce startup time and improve. Based on an entirely new design in instrumentation, the bd facsaria instrument is the first benchtop sorter that incorporates a fixedalignment cuvette flow cell. View online user manual manual for bd facscanto ii measuring instruments or simply click download button to examine the bd facscanto ii guidelines offline on your desktop or laptop computer. This is an open access protocol distributed under the terms of the creative commons attribution license, which permits unrestricted use, distribution, and reproduction in any medium. Analysis and sorting of cells with facsaria ii flow cytometer. When using facsaria mainly to analyze and sort pbmcs, since the expected size of particles are within a narrow range, is adjusting area scaling warranted each time a new experiment is set. Facsaria iiu sorp spec click on the filter for more info 355nm uv laser intercept. Manual of standard operating procedures user registration and safe working practices bd facsaria iii cell sorting in h581c. Sorting parameters ariaii please circle those required.
Is what applies to fsc area scaling also applies to blue, red and violets lasers. Fusion nozzle are label ms aria ii nozzle are label m do not switch nozzle between instruments. Equipment documentation applied precision deltavision core deltavision core specs olympus ix71 microscope stand users manual photometrics coolsnap hq2 weatherstation environmental chamber manual bd facsaria ii flow cytometer bd facsaria ii website facsaria ii tech specs bdcytopeia influx flow cytometer influx user guide isb influx quickstart operation isb influx operator manual bd. Not for use in diagnostic or therapeutic procedures. Bd biosciences application note july 2010 decontamination of the bd facsaria ii or bd facsaria iii system using the prepare for aseptic sort.
The heart of this cell sorting revolution is the true fixedalignment flow cell, gelcoupled to the collection optics. Identification and functionality of adult mouse hematopoietic stem cell side populations after enrichment on the bd facsaria ii flow cytometer equipped with a 375nm near uv laser. This video describes the process of manual and auto compensation in bd facsdiva on the lsr ii. Flow cytometry core instruments department of microbiology. And, the bd facsaria ii offers new options in lasers and nozzles to support more advanced multicolor applications. However, after some time of usage, cell sorting becomes a lot hard to set up. A product brochure and users guide are also available. Bd facsaria quick reference guide biosciences area. Facsaria ii sorter is capable of sorting 25,000 eps. New users of the bd facsaria ii flow cytometer should read. Bd facsaria quick reference guide use this quick reference guide once you are familiar with the procedures in the bd facsaria users guide. Add 250 ml of bleach to the empty waste tank if necessary. Bd facsaria fusion from bd biosciences selectscience.
All manufacturers recommended procedures in chapter 6 of the users guide will be followed. Chapter 1 to become familiar with your instrument components. Verify that the air and fluid lines are disconnected from the ethanol tank. Super soft silicone face cleanser and massager brush manual facial cleansing brush handheld mat scrubber for sensitive, delicate, dry skin 4pcs set 4. Facsaria sorting guide department of microbiology columbia. The manufacturer will be contacted before making any instrument modifications. Page 3 bd biosciences, it is the buyerusers responsibility to install and maintain uptodate virus protection. The bd facsaria iii sorter is built on the solid foundation of patented technologies, superior multicolor performance, and legendary easeofuse that opened the complex world of cell sorting to a broader audience of researchers and wider range of applications. Bd facsaria sorp operation manual 1 facsaria sorp standard operation protocol basic operation 1. The heart of this cell sorting revolution is the true fixedalignment flow cell, gel.
Biosafety and cell sorting university of washington. Bd facsaria ii, bd facsaria iiu, bd facsaria iii, bd facsaria iiiu, special order research product sorp bd. Check that the air line and the blue fluidics line is connected to the sheath tank. Fluidic lines are check daily for leaks or crimping. This protocol provides step by step instructions for performing singlecell sorting by using bd facsaria ii machine and. Turn off the lasers that are not required for analysis andor sorting.